Partial overlap of fungal communities associated with nettle and poplar roots when co-occurring at a trace metal contaminated site

Stinging nettle (Urtica dioica L.) raises growing interest in phytomanagement because it commonly grows under poplar Short Rotation Coppices (SRC) set up at trace-metal (TM) contaminated sites and provides high-quality herbaceous fibres. The mycobiome of this non-mycorhizal plant its capacity to adapt TM-contaminated environments remains unknown. This study aimed characterizing the associated with roots co-occurring a site. Plant root barcoding using fungi-specific ITS1F-ITS2 primers Illumina MiSeq technology revealed that had distinct fungal communities. was dominated by Pezizomycetes from known endophytic taxa supposedly saprotrophic genus Kotlabaea (which most abundant). Several ectomycorrhizal fungi such as Inocybe (Agaricomycetes) Tuber (Pezizomycetes) species were roots. Most present highly area whereas Agaricomycetes tended be reduced. Despite being non-mycorrhizal plant, significant proportion OTU (9.7%), suggesting some connexions between mycobiomes. Finally, our raised reconsidering networking beyond mycorrhizal interactions.